Fig. 6. Three dimensional distribution of myosin-II filaments in a live, unflattened amoeba expressing an EGFP-myosin-II tail fragment. Through a focus series of fluorescence micrographs: (A) section 1 from the dorsal surface; (B) section 3; (C) section 4; (D) section 6; (E) section 7; (F) section 10; (G) section 13; (H) section 16, ventral surface in contact with the slide. The cell was moving toward the upper left. Thick filaments are concentrated in the cortex over the dorsal surface especially the back half of the cell. Bar, 10 µm.

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