Fig. 3. (A) Amino acid sequence alignment of the kinase domains of CfNek and other NIMA-related kinases. The alignment was generated using PILEUP (GCG-package) and the output was shaded with Macboxshade. Residues conserved in >60% of the proteins are shaded in black, simililar residues are shaded in light grey. The eleven kinase subdomains are denoted above the sequence. Sequences used: Leishmania major LNK-1 (Q9NKT3), Trypanosoma brucei NrkA (Q08942), Aspergillus nidulans NIMA (P11837), Homo sapiens Nek2 (P51955), Mus musculus Nek1 (P51954) and Nek3 (Q9R0A5), Tetrahymena pyriformis TpNrk (O76134), Chlamydomonas reinhardtii FA2 (AF479588). The CfNek sequence is from Fig. 2B. (B) Phylogenetic tree derived from the alignment in A. Using the PILEUP output the tree was generated with the GCG-programs DISTANCE and GROWTREE.

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