Fig. 7. Effects of PLC inhibitors on N. crassa growth rates. (A) Neomycin treatment. (Upper panel) Control experiments for treatment with external added inhibitors show that solution exchange (BS was exchanged with BS) does not affect hyphal growth. Middle and lower panels illustrate growth rates of hyphae treated with neomycin (as marked). Neomycin inhibits hyphal growth, partially at 100 µM and completely at 400 µM. (B) 3-nitrocoumarin causes a dose dependent inhibition of hyphal elongation: complete at 40 µg/ml, very efficient at 20 µg/ml and slight at 4 µg/ml. (C) Growth inhibition with increasing concentration of U-73122. (Upper panel) Control experiments. No influence on growth rates is observed for hyphae treated with the inactive analog U-73343. (Middle and lower panel) U-73122 inhibits growth: efficiently at 10 µM and completely at 400 µM within 4 minutes after its addition. Thin lines show individual experiments. Thicker lines and symbols show means±s.e.

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