Fig. 1. Polarization of caveolin-1 during cell migration. Cell migration was induced by scraping the cells from one half of the coverslip. Primary endothelial cells were grown to confluency on glass coverslips as described. On day zero, one half of the cells on the coverslip were removed by scraping (below the yellow line) and the remaining cells were either processed directly (0 hr) for indirect immunofluorescence staining with the indicated antibody or allowed to grow for 4 and 24 hours before processing. The distribution of caveolin (left) and actin (right) are shown in the same cell. Yellow arrows point to regions high in caveolin-1 staining in migrating cells. Bar, 100 µm.

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