Fig. 4. Chromosome dynamics during late mitotic stages in an H2B-GFP-transfected PtK1 cell. H2B-GFP-transfected PtK1 cells were arrested in mitosis by nocodazole treatment and released in fresh medium for 30 minutes. From this time, late prometaphase or metaphase cells were localized and single cells were observed by fluorescence (A) and phase-contrast (B) microscopy at regular intervals. Numbers at the bottom right corner indicate the time in minutes. In the first frame (0 minutes), a PtK1 cell with all the chromosomes correctly aligned at the metaphase plate is shown; one lagging chromosome appears after anaphase onset (15 minutes) and persists as a lagging chromosome until late cytokinesis (55 minutes). Bar, 5 µm.

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