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Fig. 3. Aggregation of Saos-2 cells in response to ectopic Pax3 is Ca2+ dependent. Phase-contrast images of live Saos-2 cells infected with Ad-Pax3flag (A) in the presence of normal Ca2+-containing media and cultured for three days, or (B) 24 hours after switching the media to low Ca2+-containing media. Aggregates of Pax3flag-expressing cells dissociated after switching to low Ca2+-containing media. Bars, 80 µm.





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