Fig. 4. FGF9 induces changes in RCS cell morphology and the subcellular localization of FGFR3. (A) RCS cells were stimulated with FGF9, heparin or FGF9 with heparin for 48 hours and visualized by phase microscopy. (B) Cells were subjected to double-labeled immunofluoresent staining with anti-FGFR3 and anti-vinculin antibodies followed by reaction with a secondary anti-rabbit antibody conjugated to Cy3 and anti-mouse conjugated to Alexa 488 antibodies, respectively. Coimmunoflorescence of green and red signals identifies the sites where the two proteins colocalize. A hypass filter was used in order to emphasize staining of the focal adhesions.

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