Fig. 3. Developmental regulation of the transcripts coding for EGF-like domains containing proteins. Semi-quantitative RT-PCR analysis of transcripts corresponding to TgMIC2, TgMIC4, TgMIC6, TgMIC7, TgMIC8 and TgMIC9 genes, performed with tenfold serial dilutions of in vitro tachyzoite and in vivo bradyzoite cDNAs. To ensure that equal amounts of cDNA from each parasitic stage were being compared, the housekeeping -tubulin primers were used as controls.

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