Fig. 1. The sole fission yeast Pds5 orthologue is non-essential for viability. (A) The predicted protein product of S. pombe pds5 (SpPds5) with S. cerevisiae Pds5 (Sc Pds5), S. macrospora Spo76 (Sm Spo76), A. nidulans BIMD (An BIMD) and the human AS3 protein (Hs AS3). In each case the overall degree of relatedness to the S. pombe protein is indicated in parentheses (% identity/% similarity). Similarity between all five proteins is concentrated in the four regions filled in white, defined by the amino acid residue numbers shown above the S. pombe protein. The relatedness of each of these regions to the corresponding regions of the orthologous proteins is indicated (numbers below each conserved block; % identity/% similarity), along with the overall number of amino acid residues in each protein and the location of a predicted nuclear localisation sequence (NLS) in S. pombe Pds5. (B) Liquid cultures of HM123 (pds5⁺) and pds5 strains were grown to mid-exponential phase and were stained with Hoechst 33342 to allow visualisation of DNA and septa by fluorescence microscopy. Bar, 10 µm.

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