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Fig. 6. Rad21 and Pds5 are present in high molecular weight complexes of similar
sizes. The sizes of soluble Rad21-HA and Pds5-HA were estimated in parallel by
gel filtration chromatography as described in Materials and Methods and cell
extracts from the rad21-HA and pds5-HA strains. Samples of
each 0.5 ml Superose 6 column fraction were processed for immunoblotting with
an anti-HA antibody. The elution volume (ml) of each fraction is shown above
the blots. The positions at which dextran blue (void volume; 
2000 kDa)
and thyroglobin (670 kDa) migrated are indicated. The remaining smaller size
fractions did not contain significant amounts of either HA-tagged protein.
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