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Fig. 1. Plot of measured calcium Kd as a function of pH for the
fluorescent calcium probes furaDx (
) and OGBDx ([UNK]). The solid line
represents the predicted Kd of BAPTA using the constants
measured by Tsien (Tsien,
1980) and the methods of Bers et. al.
(Bers et al., 1994) to correct
for temperature, ionic strength and pH. All measurements were performed at
22°C with constant ionic strength (0.130 M) and variable pH (4-7.2) in
Hepes/MES pH buffers containing 1 mM Mg2+.
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