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Fig. 4. A time course showing ratiometric measurements of lysosomal pH ({circ}) and [Ca2+]lys ([UNK]) following the addition of buffer containing 10 mM NH4Cl. Lysosomes were labeled with a probe cocktail containing furaDx, FDx and OGDx. Arrow `a' indicates time of addition of NH4Cl. Arrow `b' indicates time of addition of NH4Cl-free buffer (RB) causing rapid reacidification and restoration of high [Ca2+]lys. Error bars represent the s.e.m. (n>=9 cells).





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