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Fig. 1. Time course of FBG assembly into the ECM. Confluent human foreskin
fibroblasts were incubated with 30 µg/ml Oregon-Green FBG for 1 (A,B), 6
(C,D) or 24 hours (E,F). Cells were washed, fixed and stained with
affinity-purified (to remove contaminating antibodies to FBG) rabbit anti-FN
IgG, followed by incubation with rhodamine-conjugated goat anti-rabbit IgG
(B,D,F). Panels (A,C,E) represent FBG-Oregon-Green fluorescence. Bar in (E)
represents 25 µm.
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