Fig. 8. Effect of 9D2 on FBG and FN incorporation into ECM. Confluent FN^-/- cells were treated with FN (25.8 µg/ml) and FBG (20 µg/ml) in the absence (A-C) or presence (D-F) of 35 µg/ml MoAb 9D2 or nonimmune mouse IgG₁ (G-I). Cells were washed, fixed and stained for FBG in green fluorescence (A,D,G) and FN in red fluorescence (B,E,H). Insets represent background staining for green (D) and red (E) fluorescence. Composite images shown in (C,F,I) indicate colocalization of FBG and FN in yellow-orange fluorescence. Bar in panel I represents 25 µm.

Return to article