Fig. 1. P63RhoGEF protein. (A) The human protein p63RhoGEF (GenBank accession number AXO02224) and identification by SMART method of the characteristic tandem domain, DH (for Dbl homology domain annotated by SMART as Rho GEF) and PH (pleckstrin homology domain). Segments in purple represent zones of low compositional complexity determined by the SEG program implemented in SMART (Schultz et al., 2000). (B) Multiple alignment of human p63RhoGEF DH-PH domains with other well-characterized human GEFs. Stars, double and single points indicate conserved residues, conservative substitutions and similar residues, respectively. Numbering indicates positions of start and end residues for each fragment of sequences used in the alignment. The DH domain is indicated by a solid line, while the PH domain is indicated by a dotted line. The two GEF domains of the protein Trio are displayed as h_trio1 and h_trio2 (AAC34245) (Debant et al., 1996). h_dbl (CAA21955; C. Bird, direct submission). h_tiam1 (NP_003244) (Habets et al., 1995). h_pix (NP_003890) (Nagase et al., 1995). h_duet (AB011422) (Kawai et al., 1999). h_vav (P15498) (Katzav et al., 1989). h_sos (Q07889) (Chardin et al., 1993). (C) Sequence identity dendrogram of DH-PH tandem domains obtained with the evolutionary trace method (Lichtarge et al., 1996).

Return to article