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Fig. 1. uPA induces cell polarisation, spreading and redistribution of focal adhesion complexes containing ß1-integrins and vinculin. (A,C) Unstimulated cells. (B,D) Cells stimulated with 10 nM mut C uPA for 30 minutes. (A-D) Alexa-568-conjugated phalloidin; (A,B) Anti-ß1-integrins labelled with Alexa-488-conjugated secondary antibody; (C,D) Anti-vinculin labelled with Alexa-488-conjugated secondary antibody. Bar, 10 µm. Images are representative of three experiments.





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