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Fig. 10. PI3K-independent activation of N-WASP by uPA involves its disassociation from ß1-integrin. (A,B) ß1-integrin immunoprecipitates were separated on 10% gels and immunoblotted for N-WASP. (A) Cells stimulated with 10 nM uPA for 1-30 minutes±LY 294002 (5 µM). (B) Cells stimulated with 10 ng/ml EGF for 1-30 minutes±LY 294002. The results are representative of three experiments.





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