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Fig. 5. Effect of LY 294002 on actin cytoskeleton changes induced by uPA and EGF. (A-F) Cells stained with Alexa-568-conjugated phalloidin are shown. Cells were stimulated with 10 nM mut C uPA for (A) 3 minutes, (B) 30 minutes, (D) 3 minutes + LY 294002 (5 µM; 1 hour pre-treatment) or (E) 30 minutes + LY 294002. Cells were stimulated with 10 ng/ml EGF for (C) 30 minutes and (F) 30 minutes + LY 294002. Bar, 10 µm. (G) Quantification of cells with microspikes/lamellipodia. The number of microspikes and lamellipodia were counted after 3 minutes and 30 minutes of stimulation, respectively.





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