Fig. 1. Comparison of the immunolocalisation of Pav-KLP (A,C) with the localisation of GFP-tagged Pav-KLP expressed from the poly ubiquitin promoter (B,D). (A,B) In early cysts both Pav-KLP and GFP-Pav-KLP accumulate in the ring canals that connect either the germ cells (arrow in A, large arrow in B) or the follicular cells (arrowheads in A and B) with each other. GFP-Pav-KLP is also present in germ cell nuclei (small arrow in B). (C,D) At a later stage, both Pav-KLP and GFP-Pav-KLP concentrate in the oocyte nucleus (arrowhead in C and large arrowhead in D) and persist in the ring canals (large arrow in C, not visible in D). Nurse cell nuclei accumulate much higher concentrations of GFP-Pav-KLP (arrow in D) than Pav-KLP (small arrow in C). GFP-Pav-KLP is also found along actin filament bundles from stage 10B through 11 (small arrowheads in D). Bars, 20 µm (A, B, C) and 50 µm (D).

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