Fig. 6. Overexpression of GFP-PavDEAD stabilises microtubules that have associated Orbit but not Msps protein. Fixed egg chambers expressing GFP-PavDEAD (green). DNA is in blue, and tubulin (A,D), Orbit (B,E) and Mini spindle protein (C,F) in red. (A-F) Extensive regions of colocalisation of tubulin (A) and GFP-PavDEAD are apparent and appear yellow in the merged image (D). Orbit (B,E) but not Mini spindle protein (C,F) is found along the microtubules (arrows in B,C,E,F). (G-I) Egg chambers isolated from females fed for 24 hours on unsupplemented yeast paste (G) or yeast paste containing 50 µg/ml (H) or 1 mg/ml (I) colchicine. (G) Overexpression of GFP-PavDEAD leads to a cytoplasmic network within the nurse cells (arrow) but not the oocyte (arrowhead). (H) Feeding females 50 µg/ml colchicine for 24 hours results in a partial depolymerisation of the network in the cytoplasm but not at the cortex (arrow). (I) Increasing the colchicine concentration to 1 mg/ml depolymerises the network almost completely, leaving just a thin ring of filaments around the nurse cell nuclei (arrow). Arrowheads in H and I point at the oocyte. Bars, 10 µm (E,F) and 25 µm (D,G-I).

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