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Fig. 4. Dominant-negative FGFR3 (dnFGFR3) interferes with osteo/chondrogenic development, with FGFR2 and with Brachyury expression in C3H10T1/2-BMP2. (A) Forced expression of dnFGFR3 in C3H10T1/2-BMP2 cells interferes with BMP-2-mediated development of alkaline phosphatase-positive and Alcian Blue-positive chondrocyte-like cells, respectively. (B) dnFGFR3 interferes with BMP2-dependent FGFR2 and Brachyury but not with FGFR3 expression in C3H10T1/2-BMP2 cells. FGFR3, dnFGFR3, FGFR2 and Brachyury mRNA levels in mesenchymal progenitors C3H10T1/2-BMP2 and C3H10T1/2-BMP2 with forced expression of dnFGFR3 were analysed by RT-PCR. Vector-borne transcripts encoding dnFGFR3 were analyzed by primer pairs for vector- and FGFR3-specific sequences.





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