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Fig. 4. Cells in S-phase exhibt low binding of both toxins. Cells were pretreated
with BrdU, exposed to Cy2-CTX and Cy3-ST and analyzed by fluorescence
microscopy as in Fig. 1a. Cells
were then fixed, permeabilized and analyzed for BrdU incorporation using
anti-BrdU-antibodies. Cells strongly stained for BrdU (S-phase) show little or
no binding of CTX (CTX) or ST (ST).
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