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Fig. 1. Similar opsonization of hard and soft polyacrylamide microbeads and sheets.
(a) Average fluorescent intensities of opsonized beads labeled with red
fluorescent secondary antibodies. Intensities were measured with 100 each of
stiff and soft beads from three preparations. Error bars indicate the standard
deviation between each experiment. (b,c) Images of stiff (b) or soft (c)
polyacrylamide surfaces coated with anti-BSA IgG and labeled with fluorescent
carboxylate beads coated with anti-rabbit antibodies. The average intensities
were within 7% of each other for the beads and 15% for the sheets.