Fig. 4. Late larval lineages are affected by depleting HBP expression during postembryonic development. DIC photomicrographs of postembryonic developmental stages are shown. (A) A vulva and uterus of a wild-type mid-L4 stage larva, showing the so-called `Christmas tree' morphology of the vulva at this developmental stage and obvious uterine cavity (ut). The arrow indicates the lamina that separates the uterine cavity from the vulva. (B) Abnormal vulva and uterus in a mid-L4 stage larvae fed R06F6.1 dsRNA from hatching is shown. Vulval morphology is grossly abnormal and the uterine cavity is completely absent, owing to multiple failures in the execution of uterine development. Note also the presence of germline nuclei proximal to the vulva. (C) Adult wild-type vulva. (D) Evl phenotype of adult fed R06F6.1 dsRNA from hatching. (E) Wild-type male tail, showing the spicules (arrow). Sensory rays are not visible in this focal plane. (F) R06F6.1(RNAi) male tail showing crumpled spicules (arrow). Scale bars represent 10 µm in (A,B,E,F) and 20 µm in (C,D).

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