Fig. 9. Laminin-dependent type IV collagen assembly. Cells were either untreated (a,b) or incubated with type IV collagen with (e,f) or without (c,d) laminin in the presence of anti-ß1 blocking antibody (all panels), which prevents the formation of fibrillar structures. Cells were fixed and stained as described in Fig. 8. In the absence of laminin, type IV collagen did not accumulate on the cell surface (d). In the presence of laminin, type IV collagen assembled into reticular structures (f) that co-localized with those of laminin (e). Bar, 10 µm. (g) The graph compares surface coverage by type IV collagen (gray) or laminin (black) with or without exogenous laminin.

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