Fig. 2. (A) A thin section of a parasitophorous vacuole (PVM) enclosing two replicating parasites, each tachyzoite contains two daughter buds (marked with ^*). Half of the spindle is visible in each of the mature parasites and terminates in an invagination of the nuclear membrane (enlarged insets). Scale bar=1 µm. (B) Parallel projection of optical sections obtained with a confocal microscope showing tubulin (green), centrin (red) and DNA (blue) in replicating parasites. As replication proceeds, the dividing nucleus is V-shaped, as is the spindle (arrows), and it is surmounted by the subpellicular microtubules of the forming daughter buds (arrowheads). (C) A thin section of centrioles; the centriolar microtubules are singlet microtubules rather than the triplet microtubules observed in other centrioles. (D) A thin section of daughter parasites emerging from the maternal cell. In the outward-facing areas of the daughter cells, escape involves the coordinated dissociation of the maternal inner membrane complex from the plasma membrane and association of the daughter inner membrane complex onto the plasma membrane (arrow and enlarged inset). Between the two daughter cells, scission involves membrane fusion events to create new plasma membrane (arrow and enlarged inset). Scale bar=1 µm. (E) Optical sections (1-5) and parallel projection of optical sections (P) obtained with a confocal microscope showing tubulin (green) and DNA (blue) in eight tachyzoites that have just completed budding from four maternal cells. The fully intact maternal subpellicular microtubules, apical polar ring and conoid are located at the posterior of the daughter parasites (arrows).

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