Fig. 2. Effects of mutations in yeast and Drosophila twinfilin. The most obvious phenotype of twinfilin deletion (twf) yeast strains is the random bipolar budding pattern. Wild-type diploid yeast cells (A) show a bipolar budding pattern, whereas the bud-scars, as visualized by calcofluor staining, show random distribution in twf/twf yeast cells (B). A strong hypomorphic mutation in the Drosphila twinfilin gene results in multiple morphological defects, including aberrant bristle morphology. In wild-type flies (C) the macrochaetae (arrows) are straight and show regular longitudinal groove and ridge structures. In twinfilin mutant flies (D) the macrochaetae (arrows) are often bent and show irregular surface morphology. Bars, 5 µm (A,B); 25 µm (C,D).

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