Fig. 3. F-actin structures in wild-type cells at metaphase. Green, red and blue represent F-actin, SPBs and DNA, respectively. (A) A cell in which a single leading cable extends from the aster-like structure. (B) A cell in which two leading cables extend from the aster-like structure. Rotating 3D images are shown in a and b (0° and 12°, respectively) of each panel. Direction of rotation is marked near a. (c) The F-actin cables in each cell. Thick lines represent the F-actin cables, including the aster at one side of the cell, whereas thin lines represent those at the reverse side of the cell. Deconvoluted serial sections are shown in d-o (A) or d-s (B). Pink arrows indicate an aster-like structure, and white arrows indicate leading F-actin cables. White arrowheads indicate longitudinal F-actin cable. Bars, 2 µm.

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