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Fig. 8. F-actin structures in cdc15-140 mutant cells. Green, red and blue
represent F-actin, SPBs and DNA, respectively. (A) Metaphase cell. (a) 3D
image of chromosomes and SPBs. (b,c) Rotating 3D images of F-actin structures
(0° and 24°, respectively). Direction of rotation is marked on the
right of c. (d-r) Deconvoluted serial sections around a medial region of this
cell. Pink arrows, aster-like structures. White arrows, leading F-actin
cables. (B) Anaphase cell. (a) 3D image of chromosomes and SPBs. Pink and
white arrowheads represent pairs of sister SPBs, respectively. (b) 3D image of
F-actin structures. (c-p) Deconvoluted serial sections around a medial region
of this cell. Double F-actin ring-like structures (arrows) are formed. (C) A
cell after anaphase. (a) 3D image of chromosomes and SPBs. Pink and white
arrowheads represent pairs of sister SPBs, respectively. (b) 3D image of
F-actin structures. (c-n) Deconvoluted serial sections around a medial region
of this cell. A part of the F-actin ring still remains (white arrows). Some
F-actin cables are elongated from a point (pink arrows). Bars, 2 µm.
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