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Fig. 3. Ce-emerin is not required to localize Ce-lamin, Ce-MAN1, UNC-84 or nuclear pore complexes. The panels show double-staining by indirect immunofluorescence for endogenous Ce-lamin (right panels), plus either Ce-emerin, Ce-MAN1, UNC-84 or nuclear pore complex proteins (NPCs). (A) Staining for Ce-emerin ({alpha}emerin) and Ce-lamin ({alpha}lamin) in uninjected wild-type embryos (WT; top row) and in emerin(RNAi) embryos (bottom row). (B) Double staining for each indicated marker on the left, and Ce-lamin on the right, in emerin(RNAi) embryos. Bars, 10 µm.





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