Fig. 4. RNAi-induced loss of Ce-emerin protein persists into adulthood. L1 larvae that developed from emerin (RNAi) embryos were double-stained by indirect immunofluorescence for endogenous Ce-lamin (green), endogenous Ce-emerin (red), or both (combined). The cuticle, a non-cellular structure, stained red nonspecifically. Ce-emerin was not detected in any cells or tissues examined, including the pharynx (P; upper row) and gonad (G; bottom row). The pharynx has 20 muscle cells, 20 neurons, 9 epithelial cells, and 9 specialized epithelial cells named marginal cells (White, 1988). Ce-lamin served as the positive control for antibody penetration during staining. Bar, 10 µm.

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