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Fig. 1. klp{Delta} cells have aberrant DNA segregation but normal spindle structure. Live cells with microtubules labeled with GFP-{alpha}-tubulin (green) and DNA with Hoechst 33342 (blue). (A) Wild-type cell in mid-mitosis, showing two equal masses of DNA segregating on an elongating spindle. (B) klp5{Delta} cell in early-to-mid-mitosis with DNA in three masses. (C) Diploid cell with asymmetrically segregating DNA on a long spindle. (D) klp5{Delta} cells in late mitosis with symmetric, wild-type-like DNA segregation. Bar, 5 µm.





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