Fig. 2. Chromosome segregation in wild-type and klp cells as visualized in live cells over time. The microtubules were visualized with GFP--tubulin (green), and DNA stained with Hoechst 33342 (blue). (A) Wild-type progression through mitosis from metaphase (t=0) to midanaphase B (t=9.4 minutes). (B) A klp6 mitotic cell with the DNA first as a single mass asymmetrically positioned on the spindle (t=0). The DNA initiates poleward movement (t=7.6 minutes), regresses (t=8.4 minutes), then re-initiates separation (t=9.5 minutes). (C) A klp5 mitotic cell with DNA separating asymmetrically three times (t=6 minutes; t=10.2 minutes; t=13.3 minutes), finally resulting in equal, wild-type-like segregation (t=25.5 minutes).

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