Fig. 1. ida mutants die during metamorphosis and are `imaginal discs arrested'. Wild-type (A) and ida¹⁰⁷ (B) animals are pictured at 48 hours after puparium formation. The arrow marks the position of a stalled gas bubble (B). ß-gal activity is detected in dissected tissues from esg-lacZ; ida¹⁰⁷/+ (C) and esg-lacZ; ida¹⁰⁷/ida¹⁰⁷ (D) wandering third-instar larvae. Arrows point to proliferation zones in the optic lobes that are reduced in size in ida mutants (D). Arrowheads mark the position of excorporate imaginal discs in (C), and rudimentary imaginal discs in (D). Hoechst staining of the anterior gut in wild-type (E) and ida¹⁰⁷ (F) larvae is shown. An arrow marks the presence (E) and absence (F) of the imaginal ring. ß-gal staining in the anterior gut of an esg-lacZ; ida¹⁰⁷/+ (G) and an esg-lacZ; ida¹⁰⁷/ida¹⁰⁷ (H) animal is presented. Note the severe reduction of ß-gal positive cells (arrows, H) in the imaginal ring in ida¹⁰⁷ animals. ß-gal staining in the anterior dorsal (ad) and posterior dorsal (pd) histoblast nests from esg-lacZ; ida¹⁰⁷/+ (I) and esg-lacZ; ida¹⁰⁷/ida¹⁰⁷ (J) animals are indicated. Adjacent segments are presented for each genotype. No differences are detected in primordial cell number.

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