Fig. 2. Colocalisation of LFA-1, DAF and TfR with lipid raft patches. Experimental details are as described in Fig. 1. Patches of the different cell surface markers were scored into three categories: good (>80% overlap); medium (partial but clearly overlapping regions); and none (random distribution or segregation of staining). The percentages of cells falling into each category are expressed as means±s.d. Data are representative of six experiments for LFA-1 (A), and three experiments for each of DAF and TfR (B,C). Black bars, J-ß2.7 cells expressing I-LFA-1; white bars, J-ß2.7 cells expressing wt LFA-1.

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