Fig. 4. Integrin 4ß1 has higher affinity for lipid raft patches on J-ß2.7 cells expressing I-LFA-1 than on J-ß2.7 cells expressing wt LFA-1. Cells were incubated with TRITC-conjugated Ctx-B, then crosslinked with rabbit anti-Ctx anitbody. Cells were fixed and stained with mAbs against 4ß1, followed by Alexa 488-conjugated goat anti-mouse IgG. (A) Top panels, I-LFA-1-expressing cells; bottom panels, wt LFA-1-expressing cells. (B) Colocalisation of 4ß1 with lipid raft patches. Patches of 4ß1 staining were scored into three categories as described in Fig. 2. The percentages of cells falling into each category are expressed as means±s.d. Data are representative of seven experiments. Black bars, J-ß2.7 cells expressing I-LFA-1; white bars, J-ß2.7 cells expressing wt LFA-1.

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