Fig. 6. Depletion of cellular cholesterol by MßCD reduces clustering of 4ß1 on the surface of J-ß2.7 cells expressing I-LFA-1 but has no effect on 4ß1 on wt LFA-1-expressing cells. Cells with (B,D) or without (A,C) pretreatment with 10 mM MßCD were stained on ice with the anti-4 mAb 7.2R, fixed and incubated with Alexa 488-conjugated goat anti-mouse IgG, followed by confocal microscopy. (A,B) J-ß2.7 cells expressing I-LFA-1. (C,D) J-ß2.7 cells expressing wt LFA-1. Single optical sections taken at mid-height of the cells are shown. Data are representative of two experiments. Bar, 10 µm.

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