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Fig. 8. Cytochalasin D and latrunculin A treatment affects colocalisation with lipid raft patches of {alpha}4ß1 integrin. Cells were preincubated with 5 µM cytochalasin D or 1 µM latrunculin A, then subjected to lipid raft patching and cell surface staining for integrin {alpha}4ß1 as in Fig. 7B. (A) {alpha}4ß1 integrin on {Delta}I-LFA-1-expressing cells; (B) {alpha}4ß1 integrin on wt LFA-1-expressing cells. Integrin {alpha}4ß1, green; Ctx-B, red. Single optical sections taken at mid-height of the cells are shown. Data are representative of three experiments. Bar, 5 µm.





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