Fig. 3. Changes in DAG mass and DAG species in the nucleus during cell cycle. U937 cells were synchronised by centrifugal elutriation and nuclei isolated by a method that retains the double nuclear membrane. Lipids were extracted from isolated nuclei and analysed for (A) total DAG content by DAG mass conversion, expressed as pmoles per 10⁶ nuclei or (B) DAG species by degree of saturation using silver nitrate TLC analysis, expressed as percentage of total nuclear DAG. Data shown are mean±s.e.m. of at least four separate experiments; ^*P<0.05.

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