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Fig. 4. Activation of classical PKC isoenzymes by SAG in vitro. Recombinant human PKC-{alpha}, ßII and {delta} were combined with SAG in micelle form and PKC activation measured in an in vitro assay by incorporation of [32P]ATP into histone H1 in the presence of calcium and PS. The results are presented as pmol of 32P incorporated per minute per mg PKC and are the mean of triplicates for a single experiment, representative of three separate experiments.





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