Fig. 10. A working model of class V myosins in membrane trafficking in HeLa cells. Myo5a exhibits a striking localization to small puncta of an unknown identity in the periphery. In HeLa cells Myo5b appears to associate with a major recycling compartment containing rab11a, as a dominant negative construct consisting of the Myo5b tail inhibits transferrin recycling and colocalizes with the transferrin receptor and rab11a (Lapierre et al., 2001). By contrast, the Myo5c tail associates with an apparently distinct transferrin-accessible compartment containing rab8 but not rab11a. Although the precise nature of the pathway associated with Myo5c in HeLa cells is unclear, this pathway is likely to be of particular importance in the epithelial cells where this myosin is most abundantly expressed.

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