Fig. 5. Localization of DmUba2 and DmSmt3 during cellularization, in mitotic domains, and in cultured cells. Some cells are numbered for reference in the text. (A-D) Embryos early (A,B) or late (C,D) in cellularization were stained for Pnut (red) and either DmUba2 (green in A,C) or DmSmt3 (green in B,D). (E-G, I-M) Mitotic domains in extended-germband embryos were double stained for DmUba2 (E, E inset, and green in G and G inset) or DmSmt3 (K and green in I,J,M) and either Pnut (F,L, and red in G,I,M) or tubulin (F inset and red in G inset and J). Arrowheads in I,J indicate concentrations of Smt3 in the regions of the chromosomes. Images of three different embryos are shown in K-M. (H) Clone-8 cells undergoing cytokinesis were stained for DmSmt3 (green) and Pnut (red). Bars, 10 µm (A-G, same magnification; K-M same magnification).

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