Fig. 6. p65TA increases the activity of promoters repressed by N-CoR. NIH-3T3 cells were contransfected with the above indicated luciferase reporters (A). N1-IC (0.5 µg) and N-CoR (1 µg) were contransfected in the presence or absence of p65TA (0.5 µg). Fold activation was calculated relative to cells transfected with N1-IC alone. (B) Cotransfection of p65TA (0.5 µg) with or without N-CoR (1 µg) expression vector. Cells were incubated for 12 hours in the presence of 20% FBS, and fold activation was calculated relative to cells transfected with the reporter alone. (C) Cells were transfected with a c-fos expression vector (0.5 µg), p65TA (0.5 µg), and/or N-CoR (1 µg). Fold activation was calculated relative to cells transfected with c-fos alone.

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