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Fig. 5. Regeneration of a epidermis-like epithelium includes changes in hTERT splicing. (a) Organotypic cocultures of HaCaT cells with fibroblasts after one and three weeks. After three weeks the cells have formed an stratified epidermis-like epithelium with all characteristic layers (Bar=50 µm). (b) hTERT splicing pattern of HaCaT, HaCaT-myc and HaCaT-TERT one and three weeks after plating. With regeneration of the epithelium, the full-length hTERT transcript predominates. fl, full length; {alpha}, {alpha} splice; ß, ß splice.





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