Fig. 4. Recruitment of Rab5a and syntaxin-4 to the MCPv, MCPa and SCP. Neutrophils (4x10⁸ cells) were allowed to ingest H37Rv, H37Ra or S. aureus for the indicated periods of time. Thereafter, the phagosomes were isolated and equivalent amounts of proteins were separated by SDS-PAGE and immunoblotted with anti-Rab5a or anti-syntaxin-4 antibodies. LAMP-1 was used as an internal control for the amounts of protein loaded. The blots shown are one representative of five separate experiments.

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