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Fig. 10. Cholesterol depletion affects the cellular distribution of EGFR. Untreated
HEp-2 cells (control) or HEp-2 cells preincubated with 10 mM MßCD for 15
minutes (MßCD), 1 ug/ml U18666A for 48 hours (U18666A), or 0.4 mg/ml
water-soluble cholesterol for 30 minutes (cholesterol), were biotinylated as
described in Materials and Methods. The fraction of biotinylated EGFR
(plasma-membrane-localized EGFR) was calculated. Biotinylated EGFR in control
cells was set to be 1. The figure shows pooled data from three independent
experiments (means±s.e.m.).