Fig. 2. Immuno-EM shows that binding of EGF mainly occurs outside caveolae. Ultrathin cryosections of cells incubated with EGF (10 nM) for 15 minutes on ice were double-labeled to localize bound EGF (large gold particles) and caveolin (small gold particles). In both A431 cells (A,B) and HEp-2 cells (C,D) bound EGF was found at smooth noninvaginated areas of the plasma membrane (small arrowheads), and only small amounts colocalized with caveolin in caveolae (large arrowheads). Bars, 100 nm.

Return to article