Fig. 4. Immuno-EM shows that binding of EGF occurs to EGFR both inside and outside rafts. PLAP was patched as described in Fig. 3, except that EGF (10 nM) was added together with 5 nm protein A-gold. Labeling for EGFR (A) showed that incubation with EGF on ice did not change the localization of the EGFR. Labeling for EGFR was found both outside (small arrowheads) and within (large arrowheads) rafts. Labeling for EGF (B) showed that EGF bound to EGFR both outside (small arrowheads) and within (large arrowheads) rafts. Bars, 100 nm.

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