Fig. 5. The phenotype induced by p120ctn isoforms transiently transfected into 1205-Lu melanoma cells. The expression of the FLAG-tagged polypeptides was demonstrated with MAb M2 16 hours after transfection (red signal), and nuclei were visualized with DAPI staining (blue signal). Isoforms 1A (A, B), 2A (C) and 3A (D) gave a strong signal throughout the cell, including the nucleus, and induced prominent branching phenotype (thick arrows). In some cells the signal was excluded from the nucleus (A,B,E,F; thin arrows), and these cells did not show the branching phenotype. The intermediate phenotype showed nuclear localization and lamellipodia (arrowheads in panels A,B). The isoform 4A was cytoplasmic and clearly excluded from the nucleus and did not induce the branching phenotype (E, F). Bar, 50 µm.

Return to article