Retrograde movement. The arrow points to a dynein-associated structure that was about to start a retrograde movement towards the MTOC. The same cell is shown in Fig. 4a. Time-lapse fluorescence images of dynein IC-GFP were taken in living Dictyostelium cells. All image sequences were taken every 302 milliseconds. Elapsed times are shown as 'seconds.milliseconds'. In each cell, the round, shaded region in the center corresponds to the nucleus and the bright perinuclear dynein signal colocalizes with the microtubule organizing center (MTOC).

Anterograde movement. Note the two consecutive anterograde movements (marked by arrows) along the same track, moving away from the MTOC. The same cell is shown in Fig. 4b. Time-lapse fluorescence images of dynein IC-GFP were taken in living Dictyostelium cells. All image sequences were taken every 302 milliseconds. Elapsed times are shown as 'seconds.milliseconds'. In each cell, the round, shaded region in the center corresponds to the nucleus and the bright perinuclear dynein signal colocalizes with the microtubule organizing center (MTOC).

Bidirectional movement. A single dynein-containing structure is tracked by an arrow. The same cell is used in Fig. 6a. Time-lapse fluorescence images of dynein IC-GFP were taken in living Dictyostelium cells. All image sequences were taken every 302 milliseconds. Elapsed times are shown as 'seconds.milliseconds'. In each cell, the round, shaded region in the center corresponds to the nucleus and the bright perinuclear dynein signal colocalizes with the microtubule organizing center (MTOC).

MTOC traffic I. Also see Fig. 7a. Time-lapse fluorescence images of dynein IC-GFP were taken in living Dictyostelium cells. All image sequences were taken every 302 milliseconds. Elapsed times are shown as 'seconds.milliseconds'. In each cell, the round, shaded region in the center corresponds to the nucleus and the bright perinuclear dynein signal colocalizes with the microtubule organizing center (MTOC).

MTOC traffic II. Time-lapse fluorescence images of dynein IC-GFP were taken in living Dictyostelium cells. All image sequences were taken every 302 milliseconds. Elapsed times are shown as 'seconds.milliseconds'. In each cell, the round, shaded region in the center corresponds to the nucleus and the bright perinuclear dynein signal colocalizes with the microtubule organizing center (MTOC).

MT tip traffic. The arrow points the MT plus end and the arrowhead points the signal moving in and out of the MT plus-end. Also see Fig. 7b. Time-lapse fluorescence images of dynein IC-GFP were taken in living Dictyostelium cells. All image sequences were taken every 302 milliseconds. Elapsed times are shown as 'seconds.milliseconds'. In each cell, the round, shaded region in the center corresponds to the nucleus and the bright perinuclear dynein signal colocalizes with the microtubule organizing center (MTOC).

Active dynein traffic throughout the cytoplasm. Time-lapse fluorescence images of dynein IC-GFP were taken in living Dictyostelium cells. All image sequences were taken every 302 milliseconds. Elapsed times are shown as 'seconds.milliseconds'. In each cell, the round, shaded region in the center corresponds to the nucleus and the bright perinuclear dynein signal colocalizes with the microtubule organizing center (MTOC).

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